PRRI SMART Project: An innovative technology in the field of « Epitranscriptome ».
The SMART project (2019-2021), led by the University of Montpellier and the CHU, co-directed by Prof. C. Hirtz (UM/CHU) and Dr. Alexandre David (IGF), was financed by the Occitanie region (PRRI) with European funds for regional development (ERDF funding) to the amount of 981 433€. This project includes a project leader for 3 years (Mrs. Aurore Attina) and a TQ mass spectrometer dedicated to the realization of the analyses.
This PPC-SMART project therefore aims to develop methods for the detection and quantification by mass spectrometry of RNA modifications – a unique service in France – from samples of various origins (human, animal and plant).
Currently, we detect 4 nucleosides and 26 modified nucleosides in a 30-minute LC-MRM analysis. And we continue to enhance this method with further modifications as required.
(1) MODOMICS: a database of RNA modification pathways. 2017 update
MASS SPECTROMETRY ENGINEER - SMART PROJECT MANAGER
« Hello everyone, I’m a mass spectrometry engineer and I joined PPC as the SMART project manager. My role is the development and implementation of methods for the detection and quantification of RNA changes. »
« PhD student working on RNA epigenetic : our goals are to investigate the role of mRNA methylation in colorectal cancer progression and to identify potential RNA modification-based biomarkers in cancer and neurodegenerative diseases using mass spectrometry.” ORCID