Mass Spectrometry
MRM or High Resolution, we have the solution to your problemTo carry out its research and service activities, the PPC is equipped with the latest generation LC-MS/MS mass spectrometry systems (Agilent Technologies, Bruker Daltonics and Shimadzu) as well as two biological sample preparation robots which represent an investment of 2.5 million Euros thanks to funds from the Occitania Region, mainly UM and CHU.
Multiple Reaction Monitoring
The « Multiple Reaction Monitoring» (MRM) is an acquisition method highly specific, multiplex and sensitive for selectively quantify analytes in complex matrix, and is widely used in the clinical setting for the targeted quantification of proteins or small molecules.
LCMS 8060NX Shimadzu
The development of MRM methods can be carried out on different types of matrices, ranging from biological fluids (whole blood, urine) to cell culture, for example.
High Resolution Mass Spectrometry
« High Resolution Mass Spectrometry » (HRMS) allows the identification and the characterization of thousands of proteins in complex mixtures.
timsUltra AIP Bruker + proteoElute Bruker
The timsTOF Ultra AIP, combined with Bruker’s ProteoElute solution, is a next-generation proteomics platform designed for ultra-high-sensitivity analyses. Optimized to maximize performance from limited sample amounts, this system delivers deep proteomecoverage while ensuring outstanding robustness and reproducibility.
It is particularly well suited for single-cell proteomics, low-input proteomics, and immunopeptidomics, enabling the analysisof rare samples, limited cell populations, and low-abundance peptides. This cutting-edge technology supports the mostdemanding projects in translational, biomedical, and fundamental proteomics research.
timsTOF HT Bruker + Evosep One Evosep
The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF acquisition modes including PASEF, dia-PASEF and prm-PASEF achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale.
The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.
Clinical Proteomics Platform
Institute for Regenerative Medicine & Biotherapy (IRMB)
Hôpital Saint Eloi
80 rue Augustin Fliche
34295 MONTPELLIER – Cedex 5
FRANCE